Hi,
I pulled my high titer lysates from the -80 and did a spot test and had excellent results. The spot was completely clear of the host (M. foliorum) and I took this to mean that the phage had survived the -80 storage. However, when I tried to do a titer I had no plaques on any of my plates. I tried this twice, first by picking the spot around the margins, and second by using the lysate directly. Neither produced plaques, and I used the same plates, top agar, phage buffer, and M. foliorum culture that I used in the successful spot test. I'm not sure what I'm doing wrong in the titer. Any help would be appreciated.
Thanks,
Ryan