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nic.vega posted in did you know you can do restriction digests in the microwave?
Viknesh Sivanathan posted in did you know you can do restriction digests in the microwave?
nic.vega posted in did you know you can do restriction digests in the microwave?
Covaris fragmentation
| Link to this post | posted 15 Nov, 2024 19:24 | |
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We're having a go at library prep, but the core here apparently isn't sure where to start for non-mammalian DNA. Does anyone have settings for Covaris (E220) (we're sequencing on iSeq 2x150, shooting for fragments ~400 bp)? From restriction gels, I think our genomes are all around average size for globi phage (~40-50 kb). |