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Gene or no gene?

| posted 27 Apr, 2023 14:04
We have been examining gaps in phage Culver, right near quite a few tRNAs. In one region, we find a decently long ORF(165bp). There is a smidge of CP, but very little. There is one BLAST match with Phage WilliamBoone. Should we call it? I attached an image that has a bunch of information on the gene. Thank you for any advice!
| posted 27 Apr, 2023 15:43
Hi Ellen,
I do not see any coding potential in this area. I would not call a gene there. Also remember that the red dotted lines of coding potential are not a good indicator of coding potential. (The represent patterns of 2 nucleotides (which is very noisy).
best,
debbie
 
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