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Recent Activity
Cluster G1 Gene Start
| Link to this post | posted 05 Apr, 2022 15:53 | |
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Hi we have a question concerning the start for a cluster G1 phage, GoldenAsh_1: • Glimmer calls the start at 46; GeneMark at 43. Hard to see exactly where the coding potential begins on the GeneMarkS readout. • The Starterator report does not show consensus; the most called start is at bp 43, but only in 33/93 annotated genomes. • BLASTP shows alignment from the beginning of the protein with phages containing 113 amino acids; some phages have 114 amino acids, however. Likely function: terminase, small subunit. • HHPred has a slightly higher probability with the shorter protein starting at bp 43 (92% vs. 90%). The evidence doesn’t show clear support for the longer ORF/protein, so we were going to stick with the original Glimmer call at bp 46. Any thoughts would be appreciated! I attached the annotation template with screenshots. |
3.25Mb| Link to this post | posted 05 Apr, 2022 19:28 | |
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This forum post may be useful https://seaphages.org/forums/topic/4347/ Cheers |
| Link to this post | posted 05 Apr, 2022 20:01 | |
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Hi Veronique, Thank you very much for your response! The information was useful. |