The official website of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science program.

Welcome to the forums at Please feel free to ask any questions related to the SEA-PHAGES program. Any logged-in user may post new topics and reply to existing topics. If you'd like to see a new forum created, please contact us using our form or email us at

Re-BLAST final dnam5 file

| posted 10 May, 2018 19:24
Hi -

My question is about the request to re-BLAST all features before submitting your final DNA Master files for review. We have done that for all genes in which the start site was altered as well as for all added genes. But we did not re-BLAST all genes that were called using the original auto-annotated coordinates. Is that acceptable or do you require a complete re-BLAST be done?

| posted 11 May, 2018 12:25
Hi Joe,
As long as the BLAST data matches the start chosen in the file, you are all set– no need to reBLAST.
Thanks for checking!
Login to post a reply.