The tape measure protein (Fork_Draft_54) and the tail assembly chaperone proteins (Fork_Draft_52 and 53) were identified based on blastp homology with AQ phages (Molivia_38-40). There is a slippery sequence near the end of Fork_Draft_52, TTTTTTC (as described for phage SPP1 in the Wu 2014 paper, https://www.ncbi.nlm.nih.gov/pubmed/15469818). The attached screen shot of the 6-frame translation shows this sequence; a -1 shift at 25657 would shift to the reading frame of Fork_Draft_53. This sequence is conserved in all of the cluster ED genomes.

The AQ phage genomes have a similar overall architecture to the ED phage genomes, with long direct repeats and a similar organization of structural genes. They may be helpful in identifying other ED gene functions via synteny.