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This abstract was last modified on March 16, 2021 at 12:20 p.m..

Virginia Tech

Corresponding Faculty Member: Stephanie Voshell

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GoldenSpark: A temperate phage with a troublesome genome

Morgane E McDonald, Dagen K Young, Kirin Anand, Suzette L Beeman, Sarah E Bekhet, Romina Boortalary, Rees T Bowen, Alexis K Correll, Harpreet Dhami, Samantha D Edwards, Joshua Frahm, Lexie A Goad, Matthew G Heldmann, Fadi N Hindi, James B Jorgensen, Tom R Kasputis, Allison M Lenert, Jeremy J Luers, Arya Malek, Abby E Markham, Mackenzie A Marrella, Kiley J Martin, Kaitlyn E Moise, Olivia C O'Connor, Graham D Poynter, Jayali R Samarasinghe, Troy M Scott, Rania E Smeltz, Jing G Waid, Stephanie M Voshell

Mycobacteriophage GoldenSpark was isolated at Virginia Tech in 2019 from local soil using Mycobacterium smegmatis as a host. GoldenSpark is an E cluster phage with a genome length of 75,132 bp. Several regions within GoldenSpark contain genes that were not accurately annotated through auto-annotation and required substantial manual adjustment. Analyses comparing the start positions were carefully reviewed to produce an accurately annotated genome for GoldenSpark.

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