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This abstract was last modified on March 11, 2021 at 1:06 p.m..

SUNY Old Westbury
Corresponding Faculty Member: Jillian Nissen, nissenj@oldwestbury.edu
This abstract will NOT be considered for a talk.
Annotating Akhila’s genome, a first encounter with Bioinformatics.
Joel Philip, Stephany Shaji, Umer Ahsan, Chelsea Akintunde, Mohamed Ali, Caroline Bond, Soban Choudhry, Daniel Higgins, Keisha Lacayo, Zeeshan Memon, Fernando Nieto, Jillian C Nissen, Christos Noutsos, Ramon Polanco, Anshika Rajput, Malaika Raza, Rita Reddy, Benjamin Scariah, Ariel Shalonov, Emaan Tariq

SUNY Old Westbury is a part of the 10th Cohort of the HHMI Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES). The phage Akhila was isolated in Fall 2019 as part of the phage discovery component that was integrated into the honors section of general biology I laboratory (BS2401). As this section was switched to a remote format in Fall 2020, students in this course instead annotated Akhila’s genome as a part of the SEA-PHAGES Bioinformatics project. Herein the results of Akhila’s annotation are reported. Akhila has a Siphoviridae morphotype with a temperate life cycle. It was sequenced at the University of Pittsburgh Bacteriophage Institute using Illumina sequencing with a shotgun coverage of 1484. It has 56,251 base pairs and 62.1% GC content. It belongs to the F1 cluster of Mycobacterium smegmatis mc2155 phages. Following this annotation, Akhila was noted to contain 101 genes.